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Genechem recombinant lentivirus wisp1
Recombinant Lentivirus Wisp1, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+lentivirus+wisp1/the+recombinant+lentivirus+wisp1/pm38003226-240-11-16
Average 90 stars, based on 1 article reviews
recombinant lentivirus wisp1 - by Bioz Stars, 2026-09
90/100 stars

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Transduction:

Article Title: WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway.
Article Snippet: Four concentrations of rWISP1 protein (Peprotech 120-18) were used here to intervene C28/I2 chondrocytes for 24 h (5, 20, 100, 500 ng/mL) in 6-well plates [5,29]. .. Lentiviral transduction was conducted based on three KBD patients using the recombinant lentivirus WISP1 constructed by Genechem (Shanghai, China). ..

Recombinant:

Article Title: WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway.
Article Snippet: Four concentrations of rWISP1 protein (Peprotech 120-18) were used here to intervene C28/I2 chondrocytes for 24 h (5, 20, 100, 500 ng/mL) in 6-well plates [5,29]. .. Lentiviral transduction was conducted based on three KBD patients using the recombinant lentivirus WISP1 constructed by Genechem (Shanghai, China). ..

Construct:

Article Title: WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway.
Article Snippet: Four concentrations of rWISP1 protein (Peprotech 120-18) were used here to intervene C28/I2 chondrocytes for 24 h (5, 20, 100, 500 ng/mL) in 6-well plates [5,29]. .. Lentiviral transduction was conducted based on three KBD patients using the recombinant lentivirus WISP1 constructed by Genechem (Shanghai, China). ..



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90
Genechem recombinant lentivirus wisp1
Recombinant Lentivirus Wisp1, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+lentivirus+wisp1/the+recombinant+lentivirus+wisp1/pm38003226-240-11-16
Average 90 stars, based on 1 article reviews
recombinant lentivirus wisp1 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Genechem the recombinant lentivirus wisp1
The expression of <t>WISP1</t> in KBD and control chondrocytes. ( a ) The scale bars are 500 μm and 100 μm individually. * p < 0.05. ( b ) The mRNA expression of WISP1 in KBD and control chondrocytes. * p < 0.05. n = 5. ( c ) The protein expression of WISP1 in KBD and control chondrocytes. * p < 0.05. n = 5.
The Recombinant Lentivirus Wisp1, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+lentivirus+wisp1/the+recombinant+lentivirus+wisp1/pmc10671535-161-11-16
Average 90 stars, based on 1 article reviews
the recombinant lentivirus wisp1 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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The expression of WISP1 in KBD and control chondrocytes. ( a ) The scale bars are 500 μm and 100 μm individually. * p < 0.05. ( b ) The mRNA expression of WISP1 in KBD and control chondrocytes. * p < 0.05. n = 5. ( c ) The protein expression of WISP1 in KBD and control chondrocytes. * p < 0.05. n = 5.

Journal: International Journal of Molecular Sciences

Article Title: WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway

doi: 10.3390/ijms242216037

Figure Lengend Snippet: The expression of WISP1 in KBD and control chondrocytes. ( a ) The scale bars are 500 μm and 100 μm individually. * p < 0.05. ( b ) The mRNA expression of WISP1 in KBD and control chondrocytes. * p < 0.05. n = 5. ( c ) The protein expression of WISP1 in KBD and control chondrocytes. * p < 0.05. n = 5.

Article Snippet: Lentiviral transduction was conducted based on three KBD patients using the recombinant lentivirus WISP1 constructed by Genechem (Shanghai, China).

Techniques: Expressing

The functional role of WISP1 in KBD chondrocyte damage. ( a ) The TEM results of KBD chondrocytes. Red arrows represent ( a (i)) The autolysosome in KBD chondrocyte, ( a (ii)) The mitochondria in KBD chondrocyte, and ( a (iii)) The endoplasmic reticulum in KBD chondrocyte. The yellow arrows represent a great number of liposomes. The scale bars are 2 μm, 500 nm, and 200 nm, respectively, from left to right. ( b ) The mRNA expression of selected genes in KBD and control chondrocytes. n = 5. ( c ) The protein expression of markers in KBD and controls. n = 5. ( d ) The Western blot results of markers in KBD and controls. ( e ) The bright field and dark field of the efficiency of three targets of the WISP1 lentivirus in KBD chondrocytes. The magnification is 100×. ( f ) The mRNA expression of WISP1 in KBD chondrocytes and KBD with the WISP1 lentivirus of the negative control (NC) and three targets (WISP1-A, WISP1-B, and WISP1-C). ( g ) The mRNA expression of WISP1 and other genes in controls, KBD chondrocytes, and KBD of the WISP1-A lentivirus. n = 3. ( h ) The Western blot result from markers in WISP1 knock-down KBD chondrocytes and KBD chondrocytes. ( i ) The protein expression of WISP1 and other markers in WISP1 knock-down KBD chondrocytes and KBD chondrocytes. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: International Journal of Molecular Sciences

Article Title: WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway

doi: 10.3390/ijms242216037

Figure Lengend Snippet: The functional role of WISP1 in KBD chondrocyte damage. ( a ) The TEM results of KBD chondrocytes. Red arrows represent ( a (i)) The autolysosome in KBD chondrocyte, ( a (ii)) The mitochondria in KBD chondrocyte, and ( a (iii)) The endoplasmic reticulum in KBD chondrocyte. The yellow arrows represent a great number of liposomes. The scale bars are 2 μm, 500 nm, and 200 nm, respectively, from left to right. ( b ) The mRNA expression of selected genes in KBD and control chondrocytes. n = 5. ( c ) The protein expression of markers in KBD and controls. n = 5. ( d ) The Western blot results of markers in KBD and controls. ( e ) The bright field and dark field of the efficiency of three targets of the WISP1 lentivirus in KBD chondrocytes. The magnification is 100×. ( f ) The mRNA expression of WISP1 in KBD chondrocytes and KBD with the WISP1 lentivirus of the negative control (NC) and three targets (WISP1-A, WISP1-B, and WISP1-C). ( g ) The mRNA expression of WISP1 and other genes in controls, KBD chondrocytes, and KBD of the WISP1-A lentivirus. n = 3. ( h ) The Western blot result from markers in WISP1 knock-down KBD chondrocytes and KBD chondrocytes. ( i ) The protein expression of WISP1 and other markers in WISP1 knock-down KBD chondrocytes and KBD chondrocytes. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Lentiviral transduction was conducted based on three KBD patients using the recombinant lentivirus WISP1 constructed by Genechem (Shanghai, China).

Techniques: Functional Assay, Liposomes, Expressing, Western Blot, Negative Control

The combined effect of rWISP1 with or without T-2 toxin in C28/I2 human chondrocytes. The mRNA expression of WISP1 ( a ), COL2A1 ( b ), ATG4A ( c ), ATG4B ( d ), ATG4C ( e ), BECN1 ( f ), and MAP1LC3B ( g ) in C28/I2 was influenced by the four different concentrations of rWISP1 protein with or without three different concentrations of T-2 toxin. The abscissa stands for the four different concentrations of rWISP1 protein (5, 20, 100, and 500 ng/mL). The black, green, red, and blue lines stand for 0, 2, 5, and 8 ng/mL of T-2 toxin separately. NS stands for no sense. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway

doi: 10.3390/ijms242216037

Figure Lengend Snippet: The combined effect of rWISP1 with or without T-2 toxin in C28/I2 human chondrocytes. The mRNA expression of WISP1 ( a ), COL2A1 ( b ), ATG4A ( c ), ATG4B ( d ), ATG4C ( e ), BECN1 ( f ), and MAP1LC3B ( g ) in C28/I2 was influenced by the four different concentrations of rWISP1 protein with or without three different concentrations of T-2 toxin. The abscissa stands for the four different concentrations of rWISP1 protein (5, 20, 100, and 500 ng/mL). The black, green, red, and blue lines stand for 0, 2, 5, and 8 ng/mL of T-2 toxin separately. NS stands for no sense. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Lentiviral transduction was conducted based on three KBD patients using the recombinant lentivirus WISP1 constructed by Genechem (Shanghai, China).

Techniques: Expressing